Catalog Number - 59155-01

MicroBee C18™ is a traditional octadecylsilane (C18) reversed-phase HPLC stationary phase that has been trusted by chromatographers for many years. As part of the Cogent™ Similars product line, it provides a cost-effective alternative for established analytical methods while delivering the hydrophobic retention characteristics expected from classical C18 chemistry. The end-capped, Type-A silica support is bonded with a straight-chain C18 ligand, making it especially useful for laboratories operating legacy methods and compendial procedures.

59155-01 is a 150 mm x 3.9 mm ID analytical column packed with 5 µm, 125Å MicroBee C18 particles. This dimension closely matches the format used in many historical HPLC methods developed on Microbondapak™ and similar columns, making it particularly valuable for method transfer and replacement applications. The 150 mm length provides an excellent balance of resolution and analysis time, while the 3.9 mm ID format typically performs well at flow rates of approximately 0.8–1.5 mL/min on conventional HPLC systems. The 5 µm particle size offers improved efficiency and sharper peak shapes compared to larger particles while maintaining manageable operating pressures.

This USP L1 column is well suited for hydrophobic and neutral compounds analyzed under reversed-phase conditions. The traditional C18 chemistry provides reliable retention for a broad range of analytes and is particularly effective for long-chain fatty acids, pharmaceutical compounds, and other lipophilic molecules. It remains an excellent choice for laboratories seeking a dependable alternative to legacy C18 column methods.

Key Benefits

• Traditional end-capped C18 stationary phase

• Suitable alternative for USP L1 methods

• Compatible with many legacy Microbondapak™ methods

• Higher efficiency than 10 µm particle columns

• Improved peak shape and resolution

• Excellent for hydrophobic and neutral compounds

• Effective for long-chain fatty acid analysis

• Cost-effective Cogent Similars column

Typical Applications

• USP L1 methods

• Reversed-phase HPLC

• Method transfer projects

• Pharmaceutical analysis

• Quality control testing

• Long-chain fatty acid analysis

• Lipophilic compound separations

• Routine analytical HPLC

Specifications

• Product Type: HPLC Column

• Stationary Phase: MicroBee C18

• USP Classification: L1

• Particle Size: 5 µm

• Particle Type: Type-A Silica

• Bonded Phase: C18 (Octadecylsilane)

• Endcapped: Yes

• Pore Size: 125Å

• Column Dimensions: 3.9 mm ID x 150 mm

• Length: 150 mm

• Recommended Flow Rate: 0.8–1.5 mL/min

• Brand: Cogent

Important Notes

• Designed for reversed-phase chromatography

• Traditional end-capped C18 chemistry

• 5 µm particles provide improved efficiency and sharper peaks

• Ideal for legacy method replacement

• Compatible with conventional HPLC systems

• Suitable for hydrophobic and neutral compounds

• Excellent alternative for many USP L1 methods

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